chlamydomonas reinhardtii common name
Chlamydomonas, a genus of unicellular photosynthetic flagellates, is an important model for studies of such fundamental processes as photosynthesis, motility, responses to stimuli such as light, and cell-cell recognition.C. The exposure of microalgae and plants to low UV-C radiation dosages can improve their biomass composition and stress tolerance. After scanning, the membrane was stripped and reprobed using 18S-derived probe. DNA insertion in plasmids was verified by EcoRI (NEB) digestion. Chlamydomonas reinhardtii is a single-celled eukaryote green alga that has been extensively studied as a model organism for photosynthesis, genetics, and physiology, and has been proposed as a host for molecular farming (Fields et al., 2019, Harris, 2001, Scranton et al., 2015).While several studies have investigated the use of C. reinhardtii as a food source for Daphnia species â¦ For primer extension by the Î¦29 polymerase, we used the C. reinhardtiiâspecific PETRA-T oligonucleotide 5â²-CTCTAGACTGTGAGACTTGGACTACCCTAAAACCCT-3â² (Table S1). Bacteria were plated on LB + ampicillin (100 Î¼g/ml) + IPTG (50 Î¼M) + X-gal (80 Î¼g/ml) medium overnight at 37Â°C. Related to, Vast differences in telomere length distributions in, Multimodal and very long telomeres in strains CC503+ and cw15.J14+ correspond to terminal DNA fragments. We'd like to inform you that we have updated our Privacy Notice to comply ISSN: 2575-1077 Algae in this genus have a cell wall, a chloroplast, an "eye" that perceives light, and two anterior flagella with which they can swim using a breast-stroke type motion. S Bujaldon: formal analysis, investigation, and writingâreview and editing. In 4 zoospores two may be of (+) type and two (-) type in heterothallic forms. J FulneÄek: formal analysis, investigation, methodology, and writingâreview and editing. Chlamydomonas is used as a model organism for molecular biology, especially studies of flagellar motility and chloroplast dynamics, biogenesis, and genetics. The membrane was hybridized using the Rapid-hyb Buffer protocol (GE Healthcare). 2 Î¼g of gDNA was digested in 300 Î¼l with a cocktail of six restriction enzymes (PstI, BamHI, MnlI, FokI, TaqI, and MspI; 20 units each). Prolonged culture in different growth conditions does not significantly affect telomere lengths. MT Teixeira: conceptualization, formal analysis, and writingâreview and editing. 1. Chlamydomonas is a genus of green algae consisting of about 325 species all unicellular flagellates, found in stagnant water and on damp soil, in freshwater, seawater, and even in snow as "snow algae". Nuclei were pelleted at 800 g for 2â4 min. Introduction. It has proven to be such a powerful model for dissecting fundamental processes in biology that investigators have dubbed it the 'green yeast' (Goodenough, 1992; Rochaix, 1995). The pellet was frozen at â80Â°C. Both strains were maintained on TAP agar plates or in liquid shake flasks at 25 °C with 100â150 µmol photons/m 2 /s of continuous white light. The end-labeled telomeres were then amplified with the primers 169M (poly-Gâcontaining primer) and oT1090 targeting the subtelomere/telomere junction common to 10 telomeres of eight chromosomes (Table S1). For in-gel hybridization analysis, gDNA was digested by the cocktail of enzymes following the same procedure, with some samples being pretreated with ExoT (50 units for 2 h at 25Â°C; New England Biolabs), which degrades single-stranded 3â² DNA extension and generates blunt ends, similarly to ExoI. Chlamydomonas reinhardtii Dangeard, 1899 Taxonomic Serial No. Sorbonne UniversitÃ©, CNRS, UMR 7141, Institut de Biologie Physico-Chimique, Biologie du Chloroplaste et Perception de la LumiÃ¨re chez les Micro-algues, Paris, France, Central European Institute of Technology, Masaryk University, Brno, Czech Republic, Sorbonne UniversitÃ©, PSL Research University, CNRS, UMR 8226, Institut de Biologie Physico-Chimique, Laboratoire de Biologie MolÃ©culaire et Cellulaire des Eucaryotes, Paris, France, Sorbonne UniversitÃ©, CNRS, UMR 7238, Institut de Biologie Paris-Seine, Laboratory of Computational and Quantitative Biology, Paris, France. K Riha: conceptualization, formal analysis, supervision, and writingâreview and editing. You are using a version of browser that may not display all the features of this website. One of the many striking features of Chlamydomonas is that it contains ion channels (channelrhodopsins) that are directly activated by light. Vernacular names [edit wikidata ... Chlamydomonas reinhardtiiâ (62 F) Media in category "Chlamydomonas" The following 31 files are in this category, out of 31 total. Southern hybridization was performed with a [32P]ATP-labeled (TTTTAGGG)3 probe. Scientific name: Chlamydomonas reinhardtii Dangeard : Synonym: Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941 : Former name : Common name Green alga Locality (Date of collection) near Amherst Massachusetts USA : Latitude / Longitude : Habitat (Isolation source) History: IAM (2007) Mihara, S. (1991) Someregulatory sâ¦ J Ravat: formal analysis, investigation, and writingâreview and editing. Triton X-100 was first diluted in 10 ml of buffer A per liter of culture and subsequently added drop wise to the cells while swirling them gently, to a final concentration of 0.5%. S Valuchova: conceptualization, formal analysis, investigation, methodology, and writingâreview and editing. Terminal (leaf) node. The PCR conditions were as follows: 94Â°C 3 min; 32 cycles of 94Â°C 20 s, 60Â°C 40 s, and 68Â°C 20 s; and 68Â°C 5 min. This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. The number of meiospores per zygospore are 8 in C. reinhardtii or 16-32 in C. inter-media (Fig,13 A-D, 14, 15). The authors declare that they have no conflict of interest. Plasmids were Sanger-sequenced with the M13-PU primer (Eurofins Genomics). These algae are found all over the world, in soil, fresh water, oceans, and even in snow on mountaintops. A phosphor screen was exposed to the membrane and imaged with a Typhoon FLA 9500 scanner (GE Healthcare). Chlamydomonas reinhardtiistrains CC1021 (referred to by its more common name 2137 in this article), 17Dâ(wild type in the 137c background), CC400, and CC425 were used in this study. PETRA membrane was also hybridized with [32P]ATP-labeled 1-kb ladder (Thermo Fisher Scientific). THE mating-type (MT) locus of the haploid green alga Chlamydomonas reinhardtii, located 30 cM from the centromere of linkage group (chromosome) VI, is involved in generating mating-type plus or minus gametic phenotypes in response to nitrogen starvation (G oodenough et al. gDNA from C. reinhardtii CC4350+ (â¼750 ng) was used as a control for enzyme activity and digested with 15 U of nuclease. DOI: 10.26508/lsa.201900315, Sign In to Email Alerts with your Email Address. Proteins were then denatured for 45 min at 65Â°C and DNA was extracted using 500 Î¼l of phenol:chloroform:isoamyl alcohol (25:24:1) using Phase Trap A (Peqlab). For storage, nuclei were frozen in liquid nitrogen and stored at â80Â°C. For loading controls, the gel was then transferred in denaturing conditions on a charged nylon membrane and hybridized again with the same probes. Simple, experimentally tractable systems such Saccharomyces cerevisiae, Chlamydomonas reinhardtii, and Arabidopsis thaliana are powerful models for dissecting basic biological processes. Published 3 June 2019. We thank Erin Henninger for her help in setting up the in-gel experiment. The GCM software was run by the author to fit three baramins (figure 2). 6Ã loading dye (6 Î¼l) was added prior loading onto 1.5% agarose gel. The cells were then thawed at room temperature and 5 ml of preheated buffer AP1 with RNase (QIAGEN DNA Plant Maxi Kit) was added and cells lysed at 65Â°C for 2 h. After lysis, gDNA was extracted according to the manufacturer's protocol (QIAGEN DNA Plant Maxi Kit). Full enzyme names are as follows: DXS, 1-deoxy-D-xylulose- Nuclei were resuspended in buffer B (2.5% Ficoll 400, 0.5 M sorbitol, 0.008% spermidine, 50% glycerol, and 1 mM DTT), using 1â2 ml per 200 ml of original culture volume. The current assembly of the nuclear genome is available online. Despite UV-C sharing these effects with UV-A/B but at much lower dosages, UV-C sensing and signal mechanisms are still mostly unknown. The gel was then washed three times for 30 min at room temperature with 0.25Ã SSC and imaged as for Southern blots. Roughly half of the genome is contained in 24 scaffolds all at least 1.6 Mb in length. Chlamydomonas reinhardtii is a unicellular green alga that belongs to the Chlorophytes division, which diverged from the Streptophytes division (including land plants) more than one billion years ago .It has a pair of anterior flagella, a single cup-shaped chloroplast, a nucleus, and an eyespot , and this alga shows robust circadian rhythms in many cellular processes , . Scientific name: Chlamydomonas reinhardtii Common name(s): Green algae Name as shown in Phylogenes: C. reinhardtii Ploidy: Haploid Description:. Species are listed by the code used to identify them in interspecies sequence alignments. Alternative splicing; Chlamydomonas reinhardtii; Diurnal cell cycle; Alternative splicing (AS) is a ubiquitous process that occurs in most known eukaryotes (Irimia et al. End labeling reactions (total volume 6 Î¼l) contained 100 ng of bulk gDNA, 1Ã New England Biolabs restriction buffer 4, dCTP 100 Î¼M, and 1 unit of terminal transferase (New England Biolabs, NEB) and was carried out at 37Â°C during 30 min, then 65Â°C during 10 min, and 94Â°C during 5 min. Related to, PCR verification of insertion mutants tel-m1 and tel-m2. reinhardi, the most commonly studied species of Chlamydomonas, has a relatively simple genome, which has been sequenced. Cancer and telomeres: An ALTernative to telomerase, Interstitial telomere-like repeats in the Arabidopsis thaliana genome, A genome-wide screen for essential yeast genes that affect telomere length maintenance, Role of oxidative stress in telomere length regulation and replicative senescence, Development and characterization of genome-wide single nucleotide polymorphism markers in the green alga Chlamydomonas reinhardtii, Genetic control of chromosome length in yeast, Germline replications and somatic mutation accumulation are independent of vegetative life span in Arabidopsis, Everything you ever wanted to know about Saccharomyces cerevisiae telomeres: Beginning to end, Saccharomyces telomeres assume a non-nucleosomal chromatin structure, Telomerase mechanism of telomere synthesis, Telomeres: Beginning to understand the end, Ku suppresses formation of telomeric circles and alternative telomere lengthening in Arabidopsis, Telomere length regulation in mice is linked to a novel chromosome locus, https://phytozome.jgi.doe.gov/pz/#!info?alias=Org_Creinhardtii, https://creativecommons.org/licenses/by/4.0/, Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants. Each zoospore contains neuro-motor apparatus, eye spot, two flagella and contractile vacuoles. 1984), w7 (S preitzer and M ets 1981), and No. Thank you for your interest in spreading the word on Life Science Alliance. Samples were then run in a 1Ã Tris-borate EDTA (TBE) 0.75% agarose gel in 1Ã TBE buffer for 18 h at 20 V. The gel was then dried and hybridized overnight at 37Â°C with radioactively labeled probes (oT0958, G-probe and oT0959, C-probe, Table S1) in hybridization buffer (5Ã SSC, 5 Î¼M inorganic pyrophosphate, 1 mM Na2HPO4, 5Ã Denhardtâs solution, 40 nM ATP, and 20 Î¼g/ml salmon sperm DNA). Chlamydomonas reinhardtii strain UVM4 wasgraciously provided by Prof. Dr. Ralph Bock, and npq2 (CCâ4101) was obtained by Chlamydomonas Resource Center (https://www.chlamycollection.org). MNase hypersensitivity assay was based on Lodha & Schroda (2005). Add Chlamydomonas Reinhardtii to your PopFlock.com topic list for future reference or share this resource on social media. The attractiveness of the algae as a model organism has recently increased with the release of several genomic resources to the public domain. Thus, we have described and integrated the proteometabolomic and physiological changes occurring in Chlamydomonas reinhardtiiâ¦ Exponentially growing cells (2 d in liquid culture) were gently spun and thoroughly resuspended in 90 ml buffer A per liter of culture (25 mM HepesâNaOH, pH 7.5, 20 mM KCl, 20 mM MgCl2, 600 mM sucrose, 10% glycerol, and 5 mM DTT). For specific chromosome arms, we used subtelomeric oligonucleotides 1R: 5â²-TACTTGTGTGTGCTGTGCGT-3â², 9R: 5â²-ACAGCACAATACAGTATATA-3â², and 10R: 5â²-AACGTCCTCGTGAGACCACC-3â² (Table S1). The CHSB Chlamydomonas telomere-specific oligonucleotide probe (Fulneckova et al, 2013) (5â²-GTTTTAGGGTTTTAGGGTTTTAGGGTTTTAG-3â², Table S1) was 32P-labeled at the 5â² terminus with ATP (Î³-32P) by the T4 polynucleotide kinase (New England Biolabs). Get Chlamydomonas Reinhardtii essential facts below. In brief, the membrane was prehybridized at 42Â°C in Rapid-hyb buffer for 1 h, then the radioactive probe (20 pmol) was added, and the incubation was continued for 1 h. The membrane was washed consecutively with 5Ã SSC, 0.5% SDS (42Â°C for 10 min); 5Ã SSC, 0.1% SDS (42Â°C for 20 min); and 1Ã SSC, 0.1% SDS (25Â°C for 30 min). We thank the Chlamydomonas Mutant Library Group at Princeton University, the Carnegie Institution for Science, and the Chlamydomonas Resource Center at the University of Minnesota for providing the indexed Chlamydomonas insertional mutants. Related to, Telomere length and senescence phenotype of, Genome stability in Arabidopsis cells exhibiting alternative lengthening of telomeres, Expansion of interstitial telomeric sequences in yeast, Tel1 kinase and subtelomere-bound Tbf1 mediate preferential elongation of short telomeres by telomerase in yeast, Telomere elongation chooses TERRA ALTernatives, A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length, Human intrachromosomal telomeric-like repeats: Sequence organization and mechanisms of origin, Large-scale identification of single-feature polymorphisms in complex genomes, Pinning down loose ends: Mapping telomeres and factors affecting their length, Alternative lengthening of telomeres: Models, mechanisms and implications, Genome-wide analysis to identify pathways affecting telomere-initiated senescence in budding yeast, Nucleosome positioning, nucleosome spacing and the nucleosome code, Accelerated telomere shortening in response to life stress, The dynamin-like protein Fzl promotes thylakoid fusion and resistance to light stress in Chlamydomonas reinhardtii, Telomerase-dependent repeat divergence at the 3â ends of yeast telomeres, If the cap fits, wear it: An overview of telomeric structures over evolution, Genetic architecture of natural variation of telomere length in Arabidopsis thaliana, Dynamic evolution of telomeric sequences in the green algal order Chlamydomonadales, A broad phylogenetic survey unveils the diversity and evolution of telomeres in eukaryotes, Transitions between the Arabidopsis-type and the human-type telomere sequence in green algae (clade Caudivolvoxa, Chlamydomonadales), Chlamydomonas genome resource for laboratory strains reveals a mosaic of sequence variation, identifies true strain histories, and enables strain-specific studies, Distribution of short interstitial telomere motifs in two plant genomes: Putative origin and function, Telomere length as a quantitative trait: Genome-wide survey and genetic mapping of telomere length-control genes in yeast, CST meets shelterin to keep telomeres in check, TeloTool: A new tool for telomere length measurement from terminal restriction fragment analysis with improved probe intensity correction, Fission yeast telosomes: Non-canonical histone-containing chromatin structures dependent on shelterin and RNA, Extensive restriction fragment length polymorphisms in a new isolate of Chlamydomonas reinhardtii, Isolation of a Chlamydomonas reinhardtii telomere by functional complementation in yeast, Telomeres shorten during ageing of human fibroblasts, The Chlamydomonas Sourcebook (Second Edition), Molecular analysis of telomere fusions in Arabidopsis: Multiple pathways for chromosome end-joining, Gbp1p: A protein with RNA recognition motifs, binds single-stranded telomeric DNA and changes its binding specificity upon dimerization, Chromosome end protection by blunt-ended telomeres, Telomerase and telomere-associated proteins: Structural insights into mechanism and evolution, An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii, Three Ever Shorter Telomere (EST) genes are dispensable for in vitro yeast telomerase activity, Reverse transcriptase motifs in the catalytic subunit of telomerase, Segregating YKU80 and TLC1 alleles underlying natural variation in telomere properties in wild yeast, Analysis of chromatin structure in the control regions of the chlamydomonas HSP70A and RBCS2 genes, An alternative pathway for yeast telomere maintenance rescues est1- senescence, A mutant with a defect in telomere elongation leads to senescence in yeast, Nuclear-receptor-mediated telomere insertion leads to genome instability in ALT cancers, The Chlamydomonas genome reveals the evolution of key animal and plant functions, Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes, Cell populations can use aneuploidy to survive telomerase insufficiency, Aneuploidy as a mechanism of adaptation to telomerase insufficiency, Telomerase catalytic subunit homologs from fission yeast and human, Molecular cloning and characterization of AtTERT, a telomerase reverse transcriptase homolog in Arabidopsis thaliana, How shelterin protects mammalian telomeres, Chlamydomonas reinhardtii telomere repeats form unstable structures involving guanine-guanine base pairs, A Chlamydomonas protein that binds single-stranded G-strand telomere DNA, Chlamydomonas telomere sequences are A+T-rich but contain three consecutive G-C basepairs, Replication of telomeres and the regulation of telomerase, Rap1 relocalization contributes to the chromatin-mediated gene expression profile and pace of cell senescence, Uncoupling of longevity and telomere length in C. elegans, Chlamydomonas reinhardtii as the photosynthetic yeast, Environmental stresses disrupt telomere length homeostasis, From molecular manipulation of domesticated Chlamydomonas reinhardtii to survival in nature, Establishing Chlamydomonas reinhardtii as an industrial biotechnology host, Chlamydomonas as a model for biofuels and bio-products production, Length regulation and dynamics of individual telomere tracts in wild-type Arabidopsis, Cancer. 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